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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been noticed in lots of tumors, which leads to chromosome instability, and so may perhaps lead to tumorigenesis. This protein was observed to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a vital function in marketing cell-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be explained. Two supplemental splice variants ended up claimed but comprehensive nucleotide sequence data is not really however obtainable.
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Blocking peptides are peptides that bind particularly for the goal antibody and block antibody binding. These peptide normally contains the epitope identified via the antibody.
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This manual outlines more attainable optimization of cellular and immunoassay parameters to ensure the best possible outcomes are...
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This information outlines more attainable optimization of cellular and immunoassay parameters to make sure the very best outcomes are...
Antibodies sure to the blocking peptide no more bind for the epitope on the focus on protein. By comparing the staining within the blocked antibody as opposed to the antibody alone, you can see which staining is particular.
Focus on Info Cyclin E belongs to your hugely conserved cyclin spouse and children, whose users are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins exhibit unique expression and degradation designs which lead to the temporal coordination of each mitotic function. Cyclin E sorts a complex with and functions to be a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary which is degraded as cells progress through S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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